primary antibodies against l-pgds (Santa Cruz Biotechnology)
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primary antibodies against l-pgds
![( A ) <t>L-PGDS</t> expression by WB from samples collected from MCA areas of sham-operated mice or ischemic areas at 1, 3, 5, and 7 days post-stroke. ( B , C ) WB analysis at 7 days post-stroke. L-PGDS expression levels were significantly higher in ischemic areas compared to the contralateral side of nonischemic areas. ( D – G ) Immunohistochemistry for L-PGDS and GFAP. L-PGDS was strongly detected in the leptomeninges of ischemic areas (L-PGDS [( E – G ): red]); GFAP [( E – G ): green]; DAPI [( E – G ): blue]). ( H – K ) Immunohistochemistry for L-PGDS and PDGFRβ. L-PGDS was co-expressed in PDGFRβ + pericytes in the leptomeninges of ischemic areas (( I – K ), arrows) (L-PGDS [( I , J ): red]; PDGFRβ [( I , K ): green]; DAPI [( I – K ): blue]). ( L – N ) RT-qPCR analysis. L-PGDS levels were significantly higher in brain pericytes treated with supernatant from ischemic areas ( M , N ) than in untreated pericytes (control) ( L , N ). Scale bars: ( E – G ) 100 µm; ( I ) 20 µm. * p < 0.05 between the contralateral side of nonischemic areas and ischemic areas, n = 3 for each area ( C ). * p < 0.05 between the control and supernatant–treated group, n = 3 for each group ( N ). Abbreviations: DAPI, 4′,6-diamidino-2-phenylindole; GFAP, glial fibrillary acidic protein; L-PGDS, lipocalin-type prostaglandin D synthase; MCA, middle cerebral artery; PDGFRβ, platelet-derived growth factor receptor-β; RT-qPCR, quantitative reverse transcription-polymerase chain reaction; WB, Western blot.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_5914/pmc11505914/pmc11505914__cells-13-01737-g001.jpg)
Primary Antibodies Against L Pgds, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primary+antibodies+against+l-pgds/primary+antibodies+against+l+pgds/pmc11505914-66-6-11
Average 90 stars, based on 1 article reviews
Primary Antibodies Against L Pgds, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primary+antibodies+against+l-pgds/primary+antibodies+against+l+pgds/pmc11505914-66-6-11
Average 90 stars, based on 1 article reviews
primary antibodies against l-pgds - by Bioz Stars,
2026-10
90/100 stars
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1) Product Images from "L-PGDS–PGD2–DP1 Axis Regulates Phagocytosis by CD36 + MGs/MΦs That Are Exclusively Present Within Ischemic Areas After Stroke"
Article Title: L-PGDS–PGD2–DP1 Axis Regulates Phagocytosis by CD36 + MGs/MΦs That Are Exclusively Present Within Ischemic Areas After Stroke
Journal: Cells
doi: 10.3390/cells13201737
Figure Legend Snippet: ( A ) L-PGDS expression by WB from samples collected from MCA areas of sham-operated mice or ischemic areas at 1, 3, 5, and 7 days post-stroke. ( B , C ) WB analysis at 7 days post-stroke. L-PGDS expression levels were significantly higher in ischemic areas compared to the contralateral side of nonischemic areas. ( D – G ) Immunohistochemistry for L-PGDS and GFAP. L-PGDS was strongly detected in the leptomeninges of ischemic areas (L-PGDS [( E – G ): red]); GFAP [( E – G ): green]; DAPI [( E – G ): blue]). ( H – K ) Immunohistochemistry for L-PGDS and PDGFRβ. L-PGDS was co-expressed in PDGFRβ + pericytes in the leptomeninges of ischemic areas (( I – K ), arrows) (L-PGDS [( I , J ): red]; PDGFRβ [( I , K ): green]; DAPI [( I – K ): blue]). ( L – N ) RT-qPCR analysis. L-PGDS levels were significantly higher in brain pericytes treated with supernatant from ischemic areas ( M , N ) than in untreated pericytes (control) ( L , N ). Scale bars: ( E – G ) 100 µm; ( I ) 20 µm. * p < 0.05 between the contralateral side of nonischemic areas and ischemic areas, n = 3 for each area ( C ). * p < 0.05 between the control and supernatant–treated group, n = 3 for each group ( N ). Abbreviations: DAPI, 4′,6-diamidino-2-phenylindole; GFAP, glial fibrillary acidic protein; L-PGDS, lipocalin-type prostaglandin D synthase; MCA, middle cerebral artery; PDGFRβ, platelet-derived growth factor receptor-β; RT-qPCR, quantitative reverse transcription-polymerase chain reaction; WB, Western blot.
Techniques Used: Expressing, Immunohistochemistry, Quantitative RT-PCR, Control, Derivative Assay, Reverse Transcription, Polymerase Chain Reaction, Western Blot
Related Articles
Incubation:Article Title: L-PGDS–PGD2–DP1 Axis Regulates Phagocytosis by CD36 + MGs/MΦs That Are Exclusively Present Within Ischemic Areas After Stroke Article Snippet: The membranes were incubated with primary Article Title: L-PGDS–PGD2–DP1 Axis Regulates Phagocytosis by CD36 + MGs/MΦs That Are Exclusively Present Within Ischemic Areas After Stroke Article Snippet: The brain tissue sections were deparaffinized and underwent heat treatment using a microwave for epitope retrieval in citrate buffer solution (pH 6.0; Abcam) for 10 min. Then, the samples were incubated with primary Expressing:Article Title: L-PGDS–PGD2–DP1 Axis Regulates Phagocytosis by CD36 + MGs/MΦs That Are Exclusively Present Within Ischemic Areas After Stroke Article Snippet: The membranes were incubated with primary Article Title: L-PGDS–PGD2–DP1 Axis Regulates Phagocytosis by CD36 + MGs/MΦs That Are Exclusively Present Within Ischemic Areas After Stroke Article Snippet: The brain tissue sections were deparaffinized and underwent heat treatment using a microwave for epitope retrieval in citrate buffer solution (pH 6.0; Abcam) for 10 min. Then, the samples were incubated with primary Immunohistochemistry:Article Title: L-PGDS–PGD2–DP1 Axis Regulates Phagocytosis by CD36 + MGs/MΦs That Are Exclusively Present Within Ischemic Areas After Stroke Article Snippet: The membranes were incubated with primary Article Title: L-PGDS–PGD2–DP1 Axis Regulates Phagocytosis by CD36 + MGs/MΦs That Are Exclusively Present Within Ischemic Areas After Stroke Article Snippet: The brain tissue sections were deparaffinized and underwent heat treatment using a microwave for epitope retrieval in citrate buffer solution (pH 6.0; Abcam) for 10 min. Then, the samples were incubated with primary Quantitative RT-PCR:Article Title: L-PGDS–PGD2–DP1 Axis Regulates Phagocytosis by CD36 + MGs/MΦs That Are Exclusively Present Within Ischemic Areas After Stroke Article Snippet: The membranes were incubated with primary Article Title: L-PGDS–PGD2–DP1 Axis Regulates Phagocytosis by CD36 + MGs/MΦs That Are Exclusively Present Within Ischemic Areas After Stroke Article Snippet: The brain tissue sections were deparaffinized and underwent heat treatment using a microwave for epitope retrieval in citrate buffer solution (pH 6.0; Abcam) for 10 min. Then, the samples were incubated with primary Control:Article Title: L-PGDS–PGD2–DP1 Axis Regulates Phagocytosis by CD36 + MGs/MΦs That Are Exclusively Present Within Ischemic Areas After Stroke Article Snippet: The membranes were incubated with primary Article Title: L-PGDS–PGD2–DP1 Axis Regulates Phagocytosis by CD36 + MGs/MΦs That Are Exclusively Present Within Ischemic Areas After Stroke Article Snippet: The brain tissue sections were deparaffinized and underwent heat treatment using a microwave for epitope retrieval in citrate buffer solution (pH 6.0; Abcam) for 10 min. Then, the samples were incubated with primary Derivative Assay:Article Title: L-PGDS–PGD2–DP1 Axis Regulates Phagocytosis by CD36 + MGs/MΦs That Are Exclusively Present Within Ischemic Areas After Stroke Article Snippet: The membranes were incubated with primary Article Title: L-PGDS–PGD2–DP1 Axis Regulates Phagocytosis by CD36 + MGs/MΦs That Are Exclusively Present Within Ischemic Areas After Stroke Article Snippet: The brain tissue sections were deparaffinized and underwent heat treatment using a microwave for epitope retrieval in citrate buffer solution (pH 6.0; Abcam) for 10 min. Then, the samples were incubated with primary Reverse Transcription:Article Title: L-PGDS–PGD2–DP1 Axis Regulates Phagocytosis by CD36 + MGs/MΦs That Are Exclusively Present Within Ischemic Areas After Stroke Article Snippet: The membranes were incubated with primary Article Title: L-PGDS–PGD2–DP1 Axis Regulates Phagocytosis by CD36 + MGs/MΦs That Are Exclusively Present Within Ischemic Areas After Stroke Article Snippet: The brain tissue sections were deparaffinized and underwent heat treatment using a microwave for epitope retrieval in citrate buffer solution (pH 6.0; Abcam) for 10 min. Then, the samples were incubated with primary Polymerase Chain Reaction:Article Title: L-PGDS–PGD2–DP1 Axis Regulates Phagocytosis by CD36 + MGs/MΦs That Are Exclusively Present Within Ischemic Areas After Stroke Article Snippet: The membranes were incubated with primary Article Title: L-PGDS–PGD2–DP1 Axis Regulates Phagocytosis by CD36 + MGs/MΦs That Are Exclusively Present Within Ischemic Areas After Stroke Article Snippet: The brain tissue sections were deparaffinized and underwent heat treatment using a microwave for epitope retrieval in citrate buffer solution (pH 6.0; Abcam) for 10 min. Then, the samples were incubated with primary Western Blot:Article Title: L-PGDS–PGD2–DP1 Axis Regulates Phagocytosis by CD36 + MGs/MΦs That Are Exclusively Present Within Ischemic Areas After Stroke Article Snippet: The membranes were incubated with primary Article Title: L-PGDS–PGD2–DP1 Axis Regulates Phagocytosis by CD36 + MGs/MΦs That Are Exclusively Present Within Ischemic Areas After Stroke Article Snippet: The brain tissue sections were deparaffinized and underwent heat treatment using a microwave for epitope retrieval in citrate buffer solution (pH 6.0; Abcam) for 10 min. Then, the samples were incubated with primary |